hmsc adipogenic differentiation kit  (EuroClone)

Journal: Journal of Translational Medicine

Article Title: miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

doi: 10.1186/s12967-022-03422-7

Figure Lengend Snippet: Identification of hMSCs and EVs. The hMSCs were passage-cultured. A Morphology of the fourth-passage hMSCs was observed under a microscope; B Expression of specific molecular markers CD29, CD34, CD45, CD73, CD90, CDl05, CD117 and HLA-DR was detected using flow cytometry; C Osteogenic differentiation was observed by Alizarin red staining; D Adipogenic differentiation was observed via Oil Red O staining; E Cartilage differentiation was observed through Alcian blue; F Morphology of hMSC-EVs was observed using TEM after the extraction of EVs; G Size distribution of EVs was analyzed by NTA; H Expression of CD63, CD9, CD81, HSP70 and Calnexin in EVs was measured using WB

Article Snippet: OriCell TM hMSC osteogenic differentiation kit, OriCell TM hMSC adipogenic differentiation kit, and OriCell TM hMSC chondrogenic differentiation kit were from Cyagen Biosciences (Guangzhou, China).

Techniques: Cell Culture, Microscopy, Expressing, Flow Cytometry, Staining, Extraction

Journal: Journal of Translational Medicine

Article Title: miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

doi: 10.1186/s12967-022-03422-7

Figure Lengend Snippet: hMSC-EVs suppressed OC cell proliferation, migration, and invasion. OC CAOV3/ES2 cells were treated with hMSC-EVs. A , B Immunofluorescence assay was used to detect the uptake of EVs by OC cells; C MTT assay was performed to detect the cell viability; D , E Transwell assays were employed to analyze OC cell migration and invasion. Cell experiments were repeated 3 times. Data were expressed as mean ± SD and processed by two-way (panel C) or one-way ANOVA (panels D/E). Tukey's multiple comparisons test was adopted for the post hoc test. *** P < 0.001

Article Snippet: OriCell TM hMSC osteogenic differentiation kit, OriCell TM hMSC adipogenic differentiation kit, and OriCell TM hMSC chondrogenic differentiation kit were from Cyagen Biosciences (Guangzhou, China).

Techniques: Migration, Immunofluorescence, MTT Assay

Journal: Journal of Translational Medicine

Article Title: miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

doi: 10.1186/s12967-022-03422-7

Figure Lengend Snippet: miR-18a-5p was downregulated in OC. A Heatmap of significantly downregulated miRNAs in OC microarray GSE161784 (abscissa: sample name; ordinate: miRNA name; small square: the expression level of a miRNA in a sample; upper right histogram: color scale); B Intersection of significantly reduced miRNAs in chemotherapy-resistant samples and miRNAs expressed in hMSC-EVs in the EVmiRNA database was obtained, with the middle as the intersection; C , D Expression of miR-18a-5p was determined by RT-qPCR; E Prognosis of OC patients with low and high miR-18a-5p expression was assessed using Kaplan–Meier survival analysis. Data analysis was performed using one-way ANOVA (panel C) with Tukey's multiple comparisons test for the post hoc test. The independent sample t test was used for comparisons between the two groups (panel D). *** P < 0.001

Article Snippet: OriCell TM hMSC osteogenic differentiation kit, OriCell TM hMSC adipogenic differentiation kit, and OriCell TM hMSC chondrogenic differentiation kit were from Cyagen Biosciences (Guangzhou, China).

Techniques: Microarray, Expressing, Quantitative RT-PCR

Journal: Journal of Translational Medicine

Article Title: miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

doi: 10.1186/s12967-022-03422-7

Figure Lengend Snippet: NACC1 overexpression reversed the inhibition of hMSC-EVs on OC cell proliferation, migration and invasion. NACC1 overexpression was accomplished in EVs-treated CAOV3/ES2 cells. A WB was used to detect the expression of NACC1; B MTT assay was adopted to assess cell viability; C , D Transwell assays were performed to detect OC cell migration and invasion. Cell experiments were replicated 3 times. Data were showed as mean ± SD. One-way ANOVA was performed for comparisons between groups and Tukey's multiple comparisons test was adopted for the post hoc test. *** P < 0.001

Article Snippet: OriCell TM hMSC osteogenic differentiation kit, OriCell TM hMSC adipogenic differentiation kit, and OriCell TM hMSC chondrogenic differentiation kit were from Cyagen Biosciences (Guangzhou, China).

Techniques: Over Expression, Inhibition, Migration, Expressing, MTT Assay

Journal: Journal of Translational Medicine

Article Title: miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

doi: 10.1186/s12967-022-03422-7

Figure Lengend Snippet: hMSC-EVs enhanced OC cell sensitivity to chemotherapy drugs by transferring miR-18a-5p. CAOV3 drug-resistant cell lines were constructed. A The cisplatin sensitivity of resistant cells and parental cells was compared via MTT assay; B The IC 50 value of drug-resistant cells was assessed; C The cisplatin sensitivity of cells was compared using colony formation assay; D Schematic diagram of tumorigenesis test in mice; E , F RT-qPCR was used to detect the expression of miR-18a-5p and NACC1 mRNA; G WB was employed to measure the levels of p-AKT, AKT, p-mTOR, and mTOR in cells; H Tumor volume of nude mice was detected; I , J Tumors in nude mice were separated, photographed and weighed; K HE staining was used to detect tumor nodules in lung tissues of nude mice. Independent test was repeated 3 times. Data were presented as mean ± SD. One-way ANOVA was used for comparisons between groups and Tukey's multiple comparisons test was adopted for the post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: OriCell TM hMSC osteogenic differentiation kit, OriCell TM hMSC adipogenic differentiation kit, and OriCell TM hMSC chondrogenic differentiation kit were from Cyagen Biosciences (Guangzhou, China).

Techniques: Transferring, Construct, MTT Assay, Colony Assay, Quantitative RT-PCR, Expressing, Staining